How to take perfect confocal images of U87 hepG2 ?

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Why my yeast colony PCR gel has one specific band , but the size is incorrect even when the plasmid DNA that is originally transformed is correct?

The plasmid that was transformed originally is correct (sequenced). I am expressing different gene targets from the yeast genome to optimize my protein. When I sequence the incorrect size, it...

01 July 2024 2,356 6 View

How accurate is Image J for SDS bands analysis?

I want to check my protein expression but the sample (target protein) on my SDS PAGE shows a little difference as compared to the control. I measured the band density through IMAGE J but the band...

01 July 2024 1,841 3 View

Is it possible to get hetrozygous condition in 15/15 plants in BC3F1 generation?

I made a primer that gives 2 bands in every 15 samples in ZS11xSiloga BC3F1 genration. I thought that it might be technical problem, i again ccheck it with BC2F1 generation so i got homozygous...

21 March 2024 9,382 0 View

Hello, How can i make a primer that amplify a same gene (CRa) in three different plant species?

Actually, I am trying for months to make a primer of a Clubroot resistance gene (CRa). CRa gene is found in three Brassica species. My aim is to make a primer that can amplify this one gene in all...

06 March 2024 9,877 4 View

How to fortify the food products with ginkgo biloba?

extraction techniques best food fortification from ginkgo biloba leaves health benefits seed for cultivation of ginkgo biloba plan

22 January 2024 2,303 0 View

Why does coffee has negative impact on iron absorption?

Some studies have shown coffee and other caffeinated beverages can reduce iron absorption. For example. Drinking a cup of coffee while eating a hamburger can reduce iron absorption by up to 39%....

26 December 2023 3,278 2 View

What is meant by autologous donor?

Autologous donations are blood donations that individuals give for their own use – for example, before a surgery.

26 December 2023 4,494 2 View

Why Ig G cross the placenta while others immunoglobulins cannot?

IgG is the only class of immunoglobulin capable of crossing the placenta.

29 October 2023 7,735 2 View

Request for guidance on protonation of ligand in MD simulation in gromacs and generation of correct gro file?

I hope this message finds you well. I am currently working on a research project focused on MD simulation study of drug desorption behavior on a boron nitride nanosheet with a non-covalent polymer...

28 September 2023 4,514 0 View

How to obtain correct topology of a system in which a polymer is covalently functionalized with nanosheet?

I am writing to seek assistance on a matter related to n2t file generation. I am using GROMACS and charmm36 force field in my work. I am covalently attaching a short polymer(poly-peptide) to a...

30 August 2023 8,023 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View