Iam trying to check floiurescence in bacteria, but iam failed?

I am working on E.coli bacteria after growing bacteria in LB I centrifuge it at 7000 then resuspend it and add 2 ml deionized sterile water and 19 microliters gold. In the confocal machine, there...

14 November 2019 2,774 3 View

Cell lines to evaluate cytotoxicity of drug?

Hi Everyone, I am working on some drugs for HSV. I am wondering what are the best heart, liver and kidney cells lines to evaluate cytotoxicity of my drug on heart, liver and kidney...

17 June 2019 7,591 2 View

Hi , I wanted to collect exosomes from cell culture media through ultracentrifugation.but i couldn't see pallet. Why?

I collected media in two bottles and centrifuge for 800g RCF for 10 minutes . i couldn't see any pallet , then i went for 2000g for 20 minutes still nothing what should be the possible reason?

27 December 2018 5,642 3 View

I want to collect blood of normal mouse after treating it with Ag to check drug uptake, What is best way to collect blood?

doing animal model, for cancer related research

19 December 2018 2,428 3 View

My pure anabaen sample is contaminated with chlorella vulgaris. How to get rid of chlorella and keep anabaen pure?

I am facing problem regarding purification of anabaena. i have used serial dilution and plate streaking method. But everytime the culture is contaminated with chlorella vulgaris. I have to perform...

12 November 2018 7,869 3 View

I fabricated GNP by reduction of sodium citrate but its colour is not bright red ,, what might be the reason ?

Fabrication of GNP

28 May 2018 9,702 1 View

How wind speed is mathematically related wind power???

How wind speed is mathematically related wind power???

23 February 2018 1,959 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View