Is there a high-throughput yeast crude (protein) extraction method?

More Aditi Nadkarni's questions See All

What to do for Error termination via Lnk1e in /share/apps/gaussian/g16/l502.exe, for Ce through B3LYP/SDD?

I am trying for the structure optimization via Gaussian16 DFT calculation, for a system consisting of C,H, N, O and Ce atoms. Where I am using B3LYP/ 6,311G(d,p) C, H, N and O and B3LYP/SDD for...

30 June 2024 9,979 4 View

What basis set/basis sets will be useful for DFT (using Gaussian16) of cerium ion adsorption?

24 June 2024 6,927 3 View

How to convert 100 ppm of hydrogen gas obtained from GC from a photocatalytic reactor of 150 ml containing 100 ml water?

explain the calculations

16 April 2024 5,668 3 View

What is the relation between overpotential measured for OER and Open circuit voltage ?

how to measure open circuit voltage using autolab 3 electrode system and using nova software and how open circuit voltage is related to overpotential measured for electrochemical OER

24 March 2024 2,674 0 View

Error with pdb2gmx when peptide is capped with NH2 at C terminal?

Hi , I have peptide that has NH2 at its N terminal. I am unable to perform energy minimization using CHARMM 36 force field as it is giving me error nh2 not found in residue topology. How should I...

27 February 2024 1,307 0 View

How to modify my research paper information if there is some typological mistake?

in my last latest paper on earthquake related to Turkey-Syria earthquake , the doi number is types incorrect. How do I correct this?

26 February 2024 5,843 2 View

How to find out Laccase enzyme's activity other than using ABTS?

After purifying Laccase enzyme (Bacterial origin), I tried performing enzyme assay using ABTS which is a known substrate. Still I am unable to validate its activity using the said substrate....

21 January 2024 5,159 0 View

Question about organoid immunofluorescence imaging protocol?

Hello everyone! I am new to IF imaging and I was wondering if I could get clarification on this protocol for whole organoid imaging. Below is the protocol outlined from the paper: Human bone...

15 December 2023 7,161 0 View

What do you do when your multiple regression analysis results are poor ?

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17 November 2023 5,722 3 View

Orthogonalization of time series variable?

How do I orthogonalize a time series variable? Which software and analysis can be used for orthogonalization?

01 November 2023 5,154 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

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Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How much Yeast should we add to fermentation media?

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Weak DAPI staining after immunohistochemistry - how to improve?

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The Curse of Evolution and Complexity?

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Low-yield gel extraction problem?

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Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

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04 August 2024 501 2 View

The question is how to use Wavenet transform?

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Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Natalia А Gavrilova

Aditi. For CHO lines I liked a reagent M-PER Mammalian Protein Extraction Reagent ( Thermo scientific # 78501). He maximum extracts the total protein from the cells. Varying the processing time can achieve a good result.

Christophe Cullin

We did such analysis in deep well. With 1 ml culture and NaOH/TCA precipitation, it works nicely for western blot.