I am making an RNA Seq library using ScriptSeq. This worked well the first time. But after experiencing failed libraries, I wanted to find out what stage I might be losing them at. So I conducted a QC step (using Tapestation) to see my cDNA after the terminal tagging step (before Ampure or bar code). I see peaks at 50 bp and below. Which means if I use Ampure, I will lose these small bands. I am not even sure if these small bands are cDNA or just primer dimers after cDNA synthesis. What should I do and what might be causing such low bp sizes after terminal tagging? I am doing everything as per ScriptSeq handbook. Thank you.