19 Questions 10 Answers 0 Followers
Questions related from Adam Radek Martinez
Hello Everyone, I am wanting to PCR a whole vector (except a region which I am trying to delete). The size of my desired product is around 6kb. My primers are as follows: 1. 5 -...
07 July 2019 5,437 4 View
Hello, Has anyone heard of using the Morisita Index for quantifying the stomatal arrangement? I heard it was possible but I am unsure of what it would involve. Does anyone know of a good article...
01 January 2019 486 0 View
Hello! I am interested in trying to detecting whether I have isolated a copper binding protein. I was thinking of running a native gel, then staining with copper so that the protein binds with...
11 November 2018 3,674 0 View
Hello, So I am in a bit of a time constraint. Essentially, I inserted a DNA fragment via molecular cloning which contains a unique RE site. I need to confirm whether my colony has or does not...
10 October 2018 1,734 1 View
Hello, I have successfully amplified 3 PCR products which contain overhang regions required for assembly PCR (around 25bp overlap). I tried running an assembly PCR cycle as follows: 1. From the...
09 September 2018 10,093 0 View
Hello Everyone, Recently I engineered a new fusion gene which contained a promoter region fused to a protein which is a hybrid of two proteins (genetically engineered in a certain fashion). It is...
09 September 2018 8,629 6 View
Hello, I have a substance, and I want to determine whether this substances prevents the absorption of copper within the G.I. tract. Without doing any in vivo rat work, it there a known assay that...
09 September 2018 412 0 View
Hello, So I have been doing some cloning work. and I have been comparing two gel bands. One gel band is the product before gel isolation, the other is after. On the gel, one seems bigger then the...
08 August 2018 1,170 0 View
Hello! I am looking to construct a library for cellular barcoding of hematopoietic stem cells. Are there probability based ratios I could use for determining the # possible barcodes I...
07 July 2018 2,332 2 View
I am in need of doing partial digestion for some of my cloning; however, I have fastdigist enzymes aviable and I can not find a suitable protocol for achieving partial digests with these enzymes....
07 July 2018 661 4 View
Hello, I am wanting to do overlap PCR on two fragments I have. I want to use the strategy where the overlap region acts as the primers for extending the fragment. For the overlap PCR, what...
07 July 2018 8,770 0 View
Hello Everyone, I am developing a system with two plasmids. One of the plasmids have been assigned the incompatibility group of IncH, the other is plasmid pK19mobsacB in which I am looking into...
10 October 2017 3,564 3 View
Hello, I currently have a plasmid (pFPV25.1) which contains a promoter region with multiple cloning sights in it. I would like to place a new promoter I have isolated, and I was wondering whether...
09 September 2017 3,428 4 View
Why should you use an annealing temperature that is about 5°C below the Tm of your primers? I am curious in knowing the reason for this. Does it have something to do with the inaccuracy of the...
01 January 2017 294 6 View
Hello, I am doing some bacterial transformations, and I need a GFP plasmid for reconstruction. I need to insert a new promotor region into the plasmid, and then heat shock it into an e. coli...
01 January 2017 9,890 3 View
Hello, I have come up with a new set of primers for the amplification of this one protein for a project. I am now writing, and I am not sure how I should describe the process in how I made them. I...
12 December 2016 1,564 3 View
Hello, When I grow two newly isolated bacterial strain which are silver resistant on a LB agar plate, I am noticing a zone of clearing around the colonies. These zones of clearing are Ag...
11 November 2016 869 6 View
Hello, I have these two bacteria which are silver resistant. I am wondering if the mechanism of their resistant in sporulation. However, they are able to replicate and grow on a dish and broth....
10 October 2016 4,592 2 View
DNA-binding activator / responder elements which are part of a 2 component transcriptional regulation system are a method of gene regulation. I am assuming that the DNA-binding activator proteins...
09 September 2016 753 1 View
