Hello,
I have a substance, and I want to determine whether this substances prevents the absorption of copper within the G.I. tract. Without doing any in vivo rat work, it there a known assay that can determine this?
Adam
Hello Everyone, I am wanting to PCR a whole vector (except a region which I am trying to delete). The size of my desired product is around 6kb. My primers are as follows: 1. 5 -...
06 July 2019 5,516 4 View
Hello, Has anyone heard of using the Morisita Index for quantifying the stomatal arrangement? I heard it was possible but I am unsure of what it would involve. Does anyone know of a good article...
31 December 2018 547 0 View
Hello! I am interested in trying to detecting whether I have isolated a copper binding protein. I was thinking of running a native gel, then staining with copper so that the protein binds with...
10 November 2018 3,750 0 View
Hello, So I am in a bit of a time constraint. Essentially, I inserted a DNA fragment via molecular cloning which contains a unique RE site. I need to confirm whether my colony has or does not...
09 October 2018 1,809 1 View
Hello Everyone, Recently I engineered a new fusion gene which contained a promoter region fused to a protein which is a hybrid of two proteins (genetically engineered in a certain fashion). It is...
08 September 2018 8,697 6 View
Hello, I have successfully amplified 3 PCR products which contain overhang regions required for assembly PCR (around 25bp overlap). I tried running an assembly PCR cycle as follows: 1. From the...
08 September 2018 10,164 0 View
Hello, So I have been doing some cloning work. and I have been comparing two gel bands. One gel band is the product before gel isolation, the other is after. On the gel, one seems bigger then the...
07 August 2018 1,233 0 View
Hello, I am wanting to do overlap PCR on two fragments I have. I want to use the strategy where the overlap region acts as the primers for extending the fragment. For the overlap PCR, what...
06 July 2018 8,836 0 View
I am in need of doing partial digestion for some of my cloning; however, I have fastdigist enzymes aviable and I can not find a suitable protocol for achieving partial digests with these enzymes....
06 July 2018 731 4 View
Hello! I am looking to construct a library for cellular barcoding of hematopoietic stem cells. Are there probability based ratios I could use for determining the # possible barcodes I...
06 July 2018 2,400 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?
05 August 2024 2,333 2 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View
Dear all! Oxygen vacancies do raise negatively the Fermi level by increasing the negative charge in the semiconductor crystal structure. In the case of n-type semiconductors, I wonder if this...
30 July 2024 8,339 2 View
Hello Everyone! I am trying to create a metal hydride system where I am trying to create a temperature control with the emphasis on measuring the concentration of the hydrogen with the hydrogen...
29 July 2024 7,577 2 View