In vitro assays for determining the G.I. Absorption of an element?

09 September 2018 0 434 Report

Hello,

I have a substance, and I want to determine whether this substances prevents the absorption of copper within the G.I. tract. Without doing any in vivo rat work, it there a known assay that can determine this?

Adam

Badges
Science topic

Similar topics
Geoscience
Geoinformatics

More Adam Radek Martinez's questions See All

PCR Whole Vector Issues - Methods of Optimization?

Hello Everyone, I am wanting to PCR a whole vector (except a region which I am trying to delete). The size of my desired product is around 6kb. My primers are as follows: 1. 5 -...

06 July 2019 5,313 4 View

Morisita Index for Stomatal Arrangement?

Hello, Has anyone heard of using the Morisita Index for quantifying the stomatal arrangement? I heard it was possible but I am unsure of what it would involve. Does anyone know of a good article...

31 December 2018 388 0 View

Native Gel for Copper Binding Protein Detection?

Hello! I am interested in trying to detecting whether I have isolated a copper binding protein. I was thinking of running a native gel, then staining with copper so that the protein binds with...

10 November 2018 3,581 0 View

Molecular Cloning Strategy?

Hello, So I am in a bit of a time constraint. Essentially, I inserted a DNA fragment via molecular cloning which contains a unique RE site. I need to confirm whether my colony has or does not...

09 October 2018 1,604 1 View

What is the best way of confirming the production of a fusion gene?

Hello Everyone, Recently I engineered a new fusion gene which contained a promoter region fused to a protein which is a hybrid of two proteins (genetically engineered in a certain fashion). It is...

08 September 2018 8,539 6 View

Assembly PCR Issue - Ideas?

Hello, I have successfully amplified 3 PCR products which contain overhang regions required for assembly PCR (around 25bp overlap). I tried running an assembly PCR cycle as follows: 1. From the...

08 September 2018 9,999 0 View

Gel Extraction Products can seem larger on a gel?

Hello, So I have been doing some cloning work. and I have been comparing two gel bands. One gel band is the product before gel isolation, the other is after. On the gel, one seems bigger then the...

07 August 2018 1,083 0 View

Partial Digestion with FastDigest Enzymes?

I am in need of doing partial digestion for some of my cloning; however, I have fastdigist enzymes aviable and I can not find a suitable protocol for achieving partial digests with these enzymes....

06 July 2018 561 4 View

Recommended Library Size For HSC Cellular Barcoding?

Hello! I am looking to construct a library for cellular barcoding of hematopoietic stem cells. Are there probability based ratios I could use for determining the # possible barcodes I...

06 July 2018 2,233 2 View

Recommended Overlap PCR Protocol?

Hello, I am wanting to do overlap PCR on two fragments I have. I want to use the strategy where the overlap region acts as the primers for extending the fragment. For the overlap PCR, what...

06 July 2018 8,653 0 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View