Basically i prepared PLGA based nanoparticles by QbD approach. As Zeta potential is a Critical Quality Attributes(CQA) i have to explain the effect of CMAs and CPPs on CQA. Due to PLGA polymer, the value of Zeta potential was found to be negative. What are the other CMAs and CPPs which can influence the value of zeta potential(CQA)?
Goutam Kumar Jena Zeta potential (ZP) is a property of the material/particle and the medium it sits in. Thus it's not simply a function of the chemistry of the surface of the material but is a function of external environment especially pH but also ionic concentration and presence of polyvalent ions such as Ca2+, Al3+, Fe3+ etc. BTW ('by the way'), you define one acronym (CQA) but fail to define others (PLGA, CMA, CPP). Some will be easily recognized (e.g. QbD) but you should be consistent. A little bit of work on Google Scholar will assist you.
Goutam Kumar Jena Zeta potential (ZP) is a property of the material/particle and the medium it sits in. Thus it's not simply a function of the chemistry of the surface of the material but is a function of external environment especially pH but also ionic concentration and presence of polyvalent ions such as Ca2+, Al3+, Fe3+ etc. BTW ('by the way'), you define one acronym (CQA) but fail to define others (PLGA, CMA, CPP). Some will be easily recognized (e.g. QbD) but you should be consistent. A little bit of work on Google Scholar will assist you.
Zeta potential is influenced by both solute and solvent factors.
Goutam Kumar Jena You say zeta potential is a CQA in your application. Have you demonstrated that or is it a pCQA (potential CQA)? Be aware that "zeta potential" is just a number calculated from electrophoretic mobility (assuming light scattering) and the latter is the true attribute the instruments measure. The problem with using zeta potential instead of electrophoretic mobility is that it constrains your explanation of changes in mobility because zeta potential calculation uses models that simply may not be valid. It's also a problem because different instruments may use different models and not clearly indicate which.
For QC needs, I recommend using electrophoretic mobility. But you have to educate your colleagues and get them away from the mindset of zeta potential.
Also, it is possible that variations zeta potential/mobility are a result of changes in a different attribute that are the cause of failure.
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