Zein Protein Solubilization?

Ramiro Galleguillos @Ramiro-Galleguillos

24 June 2016 1 5K Report

I am trying to prepare a saturated solution of Zein protein in a SLS aqueous surfactant solution, ~200mM. We add plenty of Zein. We obtain an opaque colloidal dispersion. How do we filter/centrifuge the dispersion to remove excess Zein and obtain a clear liquid?

Yuri Mirgorod

Dear Ramiro Galleguillos:

Zein amino acids interact with the ionic SDS. Therefore separate them in the aqueous solution difficult. Determine the isoelectric point of the protein + SDS of method DLS. Create this pH in the solution. Interact between Zein + SDS weaken and protein should be precipitated or centrifuge.

Best regards.

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