Can mycoplasma contamination in primary neuronal culture interfere with neuronal growth?

I am doing primary neuronal cultures (mix of neurons and astrocytes) from mice. One year ago we moved and changed labs and since that time I am not able to grow nice neurons (either they don't...

30 September 2020 2,517 1 View

Does anyone have experience with the Menstrual Attitude Questionnaire?

I am considering of using the original one from Brooke-Gunn and Ruble (1980), but maybe there is something better? I am trying to analyze the attitude of athletes towards menstruation and its...

27 August 2020 6,541 2 View

The compensation procedure- beads (for fluorochrome) and cells (dead/live staining)- how to do it?

Hi, I would like to ask about the compensation procedure. I use Fortessa/ Diva and automated compensation procedure. I have a set from BD for compensation setting. Referring to the manufacturer...

28 February 2020 8,765 2 View

What standardised psychometric test to use for self-efficacy for female athletes?

Hello, I am looking for a valid and standardised test to measure self-efficacy of female athletes for my doctoral thesis. Any suggestions? Thank you

14 November 2019 849 3 View

What is the best recommended growth and freeze media for SH-SY5Y cells?

I received SH-SY5Y WT cells and started growing them in low glucose DMEM with FBS and S/P. After reading article about changed cell morphology and detachment of cells from growth surface due to...

06 October 2019 1,475 2 View

Why can't I load my purified pcr sample from a gel to another gel to confirm purification The product evaporates out of well before I can run the gel?

23 July 2018 3,700 8 View

Why all phages in ELISA are positive?

I perform 5 biopannings rounds and then did ELISA with the 'soup of phages' from each circle (coating with specfic antybody, then phages and antiM13). Biopannings from 1-4 had really low OD but...

18 June 2018 915 3 View

Can someone help me determine the NMR data for 2,5-dimethoxyampehtamine?

Interpretation of this compound and all the chemical shifts

20 March 2017 703 1 View

Does anybody has an experience with human serum depletion prior to MS- or electrochemical analysis?

12 March 2015 8,710 5 View

Does anyone use a thermo-desorption technique for mercury speciation in solid samples?

22 February 2015 9,352 11 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

How Can I clain a removal of a co-author?

I would like to make a correction in a co-author in your paper which is wrong. This name is also in other papers of Kenton Ko. Zey Ko is not a Co-author . The correct name is Zdenka...

24 February 2021 3,476 4 View

Is it mandatory to specify Subjects Combinations in Generalized Linear Mixed Models (SPSS)?

Hi all, I have data with two levels: lets think of the classic example of students and schools. When running the analysis in SPSS and selecting the Random Effects, we can select (we can or we...

24 February 2021 7,379 4 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View