Writing user subroutines in ABAQUS?

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Cloning of flaviviruses is difficult i have noticed that cells loose their transformation ability after cloning fragments inside vector any advice?

As flaviviruses genome is very frequently prone to mutation hence it is very difficult to clone whole genome inside vector what kind of vector and cells can sustain such amount of cloning...

11 July 2024 4,284 0 View

I am not getting proper colonies while ligating one of fragments in my vector the colonies seems to be normal but in light it seems lysed any reason?

I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...

11 July 2024 9,010 3 View

How can I plot DOS till 20 eV please tell me?

I have made changes in .in1 as well as in .int files but unable to get dos till 20 eV

13 June 2024 9,080 1 View

Why generally we prefer phenol chloroform extraction method for DNA purification as i always face difficulty in getting good concentration why?

phenol chloroform extraction method is used for purification of nucleic acids DNA/RNA

03 April 2024 8,268 0 View

After phenol chloroform extraction the nucleic acid concentration is around 20ng/ul but after loading 1ul/2ul its still not visible why?

phenol chloroform extraction method is used to purify nucleic acids to obtain a good concentration.

03 April 2024 7,244 0 View

Can anyone suggest some specific bacterial cell line for cloning viruses, specifically flaviviruses i have used cell like TOP-10/DH10B/DH5 suggest?

as viruses are notorious for being prone to mutation at high rates its very difficult to clone them though general cloning procedures and cells so in order to solve this problem need specific cell...

03 April 2024 9,031 5 View

Why we have two differnt formulas for the calculation of seebeck coefficient in theoretical and experimental methods?

For the boltaztrap we used the formula for calculating the Seebeck coefficient using a different formula mentioned in doi:10.1016/j.cpc.2006.03.007 and the formula we are using in the experiment...

17 March 2024 2,105 3 View

How much "instant" a photon is?

There are two rooms: Room A and Room B; initially both are dark; contains no light source (of whatsoever). Now, I bought a light source and set it up in the room A, enabling me to see things...

28 February 2024 4,809 10 View

What are R packages for detecting peaks and peaks area for HPLC chromatogram?

I am performing snake venom fractionation using Reverse-phase HPLC. It generates multiple peaks with different area under the curve. Since I'll be analyzing multiple chromatograms at different...

08 November 2023 9,134 0 View

Looking for an opportunity to visit any of the campus in the United States working in the area of biotechnology. Please respond and can you help?

Dear All, Biotechnologists from The area of Biotechnology. Currently I am serving as HoD in Department of Biotechnology, IMSEC, New Delhi NCR. Is there any person who can help me for two or three...

08 November 2023 2,785 4 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

Can anyone explain why there is not any color bands in the middle portion of the tensile coupon?

Details of the Analysis. Static Analysis Composite Layup Continuum Shell Elements FRP Material (Elastic and Hashin Damage)

03 August 2024 8,538 4 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Simulation of metal drawing by Abaqus with UMAT?

Hello, colleagues. Recently, I have been working on a metal processing simulation with my UMAT in Abaqus. I have outlined the corresponding simulation, but I keep encountering issues that cause...

30 July 2024 7,062 1 View

PhD thesis topic?

I want to write a topic for my PhD thesis in hospitality (hotels), can u please suggest some variables

29 July 2024 9,058 3 View

What are the main types of errors and causes that English major college students commit in their English writing?

a 15-page paper

29 July 2024 1,956 5 View

Why running a restart analysis in Abaqus in Ubuntu OS gives an error as attached when running the same job in Windows doesn't give any error and runs?

I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...

29 July 2024 9,572 3 View

Ronald Heinz Norbert Wagner

I am giving ABAQUS Subroutine Lectures here:

https://youtube.com/playlist?list=PL4qG7zaS9ome3teA3h3dMMEwEToKloDlv

https://www.researchgate.net/project/Creating-SUBROUTINES-In-Abaqus-Lectures-by-Dr-Ronald-Wagner

future lectures will come later this year

Srinivasan Echchur Rangarajan

Yes. Yoy can DM me in case you need some reflection.

Ma Mingze

I wrote some subroutines concerning fatigue several years ago, the most important thing is defining damage related to loading cycles