How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

How to Implement Elastoplasticity in a PCL model in COMSOL Multiphysics?

I am using COMSOL Multiphysics 5.5. I am trying to simulate the tensile test of PCL scaffolds. However, regardless of the strain, I have the elastic region all the time which is not true when it...

23 February 2021 4,050 1 View

Would bisulfide sequencing result be affected if the tissue is fixed by perfusion?

I am looking into DNA methylation of mice as an aging marker along with other assays and recently I added brain IF to the list of assays. For this, I need to fix the brain by transcardial...

09 February 2021 9,012 3 View

Why E1g mode (Molybdenum disulfide) is undetectable in Raman spectroscopy?

I synthesized 1T/2H hybrid phase MoS2 on a graphite foil surface by hydrothermal method, and then analyzed it by Raman spectroscopy. As a result, three new peaks correspond to the 1T phase were...

04 February 2021 6,580 2 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View

Where can I find these two papers?

14 January 2021 1,572 3 View

What form of data analysis should I use for my pilot study?

I am currently conducting my final year dissertation which is looking at the efficacy of common police lineup practices. However, before I launch my study, I have put out a pilot study which is...

08 January 2021 4,376 4 View

Would it make difference in HSC ratio if I cryopreserve bone marrow?

I collected bone marrow from mice to be analyzed for hematopoietic stem cell (HSC) ratio, and due to circumstances, I could not run flow cytometry the same day. So I have cryopreserved bone marrow...

07 January 2021 5,559 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Your thoughts on the spherical densiometer to estimate relative species canopy cover?

Hi biologists and biogeomorphologists, I want to characterize vegetation plots on a transect that follows a successionnal gradient from a river bed to a mature forest. Ecologically, I am...

26 February 2021 5,720 2 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

Why should we use Sustainable climate design?

Sustainable design is a set of cognitive and analytical abilities, knowledge of the environment and ample practical to incorporate things into the world of microbes, plants and animals, and the...

25 January 2021 7,816 8 View

What can possibly cause only negative droplet generation in ddPCR reaction?

Dear all, I have started experiment with ddPCR using previously established protocol on methylation analysis. However, my first run did not work well. I could only see a negative droplets in all...

05 January 2021 8,575 2 View

Spatial Panel Diagnostic Test?

Hello Colleagues Trust you are all fine, Please I have been struggling for days on estimating some spatial diagnostic test on my model. The stata command I am using is spatdiag command. I am...

04 January 2021 3,919 1 View

Is there any good online workshop on advanced DEA models?

OR ANY study material with step by step analysis & its interpretation using the software?

01 January 2021 993 1 View

Where would money flow into, if it was suddenly announced that it would become worthless after 48 hours?

Silvio Gesell proposed the idea of FreeMoney, which posited that money loses value over time. As such, this can accelerate circulation, as no one would want to bear the cost of holding on to...

25 November 2020 7,555 8 View