How do you multiple impute item scores and perform a linear regression on factor scores?

I am developing a questionnaire and first performing an exploratory factor analysis. After I have the final factor structure, I plan on regressing the factor scores on some demographic covariates....

18 January 2021 883 8 View

How to remove dried cells/DNA off a nitex membrane for DNA extractions?

I'm working on developing a method to extract and isolate DNA from respiratory condensate or "blow" samples collected from beluga whales. In the field, we tried 2 different collection devices, a...

28 August 2018 4,866 2 View

Why are my SYBR green standard curve dilutions not amplifying and have flat curves?

Please help! Recently our SYBR Green qPCR reaction using our housekeeping gene, RPLP0, and all the same conditions stopped working one day to the next. We isolate RNA from tissue culture human...

03 August 2017 2,418 13 View

How to extract enough RNA from jellyfish polyps (Aurelia aurita) to monitor the expression variations of some genes (RT-PCR and qPCR)?

I would be very grateful if the community could give me some tips: as these organisms are very small, I wonder how many polyps do I have to pool for one RNA extraction or if one is enough? What is...

29 January 2017 4,605 2 View

Does anyone know what the average cell diameter of Panc1 cells is?

27 November 2016 5,872 3 View

In case of infected root samples, is the infection stopped by the Gus solution or by the ethanol washing bath (+ ref please :) )?

In my case, I'm working with entire root systems of Medicago truncatula infected by an oomycete.

18 October 2016 2,471 4 View

Why can I not digest or sequence my plasmid?

I amplified a plasmid and now I have an eluted stock from a maxi prep i did. I sent the plasmid out for sequencing and it came back as no priming. I tried a restriction digestion with 2 different...

13 August 2015 4,170 6 View

Any suggestion on the seeding density for primary monocytes in a 96-well?

I am doing a one-day assay with primary monocytes and seeding them at 3*10^5/well, as per a colleague recommendation. However I see others recommend much lower, but they seem to have different...

03 June 2015 6,422 2 View

How do I stimulate cell cultures with beta-estradiol?

I have been doing cell culture experiments that involve treating the cells with estradiol (E2). There are many papers published that state these cells are responsive to estrogen. The problem is...

15 May 2015 2,953 6 View

Can I use DataAssist or ExpressionSuite software for analysis if I acquired my qPCR data on the LightCycler 480?

As opposed to using Excel for analysis.

23 January 2015 5,201 1 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

No title

02 March 2021 3,060 3 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

Can anyone recommend which is the best free questionnaire software please?

Hello, I am looking to send out links to an online survey. My previous research project used a paid for survey software. I am now looking for something that's free but straight forward to...

19 February 2021 5,641 3 View

Does qualitative research using closed and open ended survey and semi structured interviews qualify as triangulation?

I am using qualitative research to look at employee motivation. I will use a closed and open ended survey (for biodata information and general views to motivation) and semi structured interviews...

18 February 2021 436 11 View

Should I include less important items in con-joint survey experiment?

I plan to do a conjoint survey experiment in which I randomly change attributes of hypothetical job candidates and show two types of potential candidates with different attributes. Then, I ask...

16 February 2021 5,812 1 View

How can we measure the reliability and validity of our survey questionnaire?

We are conducting survey research on COVID-19 misinformation among students. How can we validate our survey for it to produce valid and reliable data? Also, what statistical tools can we use to...

16 February 2021 678 3 View