I've not worked with these cells before but there are basics to cell culture that can be applied to your question...

Normally the culture collection that you obtained the cells from will indicate what growth conditions are. ATCC states BEGM.

Although most cells could grow in DMEM, DMEM comes in different makeups - high/low/no glucose, high/low/no glutamine, with/without sodium pyruvate, with/without sodium bicarb. You could try matching the ingredients that make up BEGM but then why not just buy BEGM in the first place?

Another consideration is whether the cells are differentiated and if BEGM helps or maintains cellular differentiation. Most likely if you grow the cells in standard DMEM you may lose this differentiation. You also need to consider whether using DMEM to BEGM will cause expression of different genes or change the morphology/function of the cell line.

If you still want to press ahead, just split a spare flask and add in DMEM to see what happens!

Hope it helps!

Hi

As far as i know  bronchial epithelial growth media (BEGM) is used for culturing  THLE2 cells. DMEM  also can be used but better to use BEGM.

Thanks and regards,

Mamatha

Hello

Yes I agree with mamathat , you can use  DMEM but the best of this cell is BEGM . 

https://www.lgcstandards-atcc.org/products/all/CRL-2706.aspx?geo_country=tr

Thank you everyone...I am currently using BEBM (Lonza) and the cells are growing well..!!

Hi,

Does someone work with THLE-2 cells using the following medium from ATCC?

PCS-300-030 Airway Epithelial Cell Basal Medium

PCS-300-040 Bronchial Epithelial Cell Growth Kit      

I have some doubts, because it is not possible to exclude ephinefrin from the bullet kit.

Thanks,

Simona

Hello,

I am planning to work on THLE-2 cells and I have the necesasary media for it. My question is: Did you pre-coat your flasks like ATCC recommended or not ?

Thanks

Hi Malak,

yes, we pre-coat flask following the ATCC protocol.