I'd appreciate it if anyone could show me the right direction. I've been working lately for straight two years with gas chromatography. I know HPLC a little, but I'm not that familiar with high perfomance liquid chromatography. Although, my aim right now led me to find the best possible way to measure the quantity of biognic amines. I thought to myself that maybe I'd try with GC, however, a colleague of mine advised me to also consider HPLC. Shall I pick one of them or neither one would be proper for this aim?
HPLC would be better option. RP-HPLC can be used with Methanol-water as mobile phase with VWD/DAD detector.
In addition to colleagues notations , the molecular weight of your sample is consider an important matter to select the separation methods .
Im also of the same opinion. HPLC would be better choice as molecular weight of your molecules is expected to be high thus HPLC moreover amines may not be volatilized easily thus GC not recommended. You can use RP-HPLC using suitable solvent system and detector. If required you can extend your studies on LC-MS-MS for further characterization.
Hi Szymon, although I did not work on biogenic amines before, I can share one story with you. One student in our lab studied biogenic amines in soy sauce, and she compared HPLC and GC-MS methods before the study, finally, she used HPLC. I hope it could help you somewhat.
Thank you all for your responses, I think I've made my mind up. By now, I'm pretty sure I will have a go with a HPLC method. I was thinking about GC-MS due to the fact I used GC a lot in my previous studies and I thought to myself it would work here too, as based on my experience and some of the publications. Thanks once more!