In the course of sample preparation for an in vitro antioxidant assay using the ethanolic seed extract of Piper guineense, I discovered that the extract was not soluble in water.
Ayomikun Joshua Pirisola Sir,
DMSO is considered a universal solvent. You can dissolve the plant extract in DMSO. It would not affect the activity of plant extract.
I also used the same for my plant extracts. You can go for this.
Regards
Preety Rohilla
Thank younfor your reply. I read that DMSO can impair with the plant extract which might lead to a kind of oncogene formation
Since DMSO is used as a universal solvent, the effects on the plant activity should be minimal (infinitesima). However, to be on safer side, the total moisture can be removed from the extract before the analysis is done.
Not really but you can actually use lyophilizer to remove the moisture content before you start your normal analysis on the extract. No need to perform the moisture analysis because the aim is not to determine the amount of water but to ensure that the moisture is totally removed.
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts