So far I have only performed migration assays with suspension cells and now I am trying to set up the migration assay with murine macrophages, differentiated from bone marrow cells, in a Corning Transwell System. I am wondering for instance, if I need to seed the cells into the upper chamber on the day before the assay so that they have some time to recover after harvesting and to adhere. What is the best way to stain the migrated cells? Crystal violet or DAPI or any other recommendations?
I would be grateful for any tips and tricks!
I had previously performed transwell cell migration assay with DAPI staining and it worked well. But be aware of the pore size for the murine macrophages.
I think seeding cells into the upper chamber on the day before assay may be depending on the purpose of your assay and manupulation. But I normally did not perform such kind of protocol, and i'm not sure whether this would negatively impact the assay or not.
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