When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

SPSS - Merging files by ID numbers that contain letters?

Hi, I'm trying to merge files from a data base of 20 files, each containing about 4000 subjects. Unfortunately the ID numbers are actually a combination of numbers and letters (e.g., b1458219)....

15 February 2021 5,833 3 View

How would I go about getting an expert review on the interview questions for the dissertation?

Good Evening, My name is David Marshall and I am Doctor of Management Student at Colorado Technical University Online. I just recently received approval from the IRB to start data collection on...

08 February 2021 346 7 View

How can I quantify the color of a solution?

I know colorimetry has been the standard way by way of measuring the concentration of a particular compound (solute) in a colored solution (solvent or measure quantities of a particular compound...

08 February 2021 2,673 1 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

How does DMSO percentage affect ELISA?

Hello, In my sandwich-type ELISA which is testing for small molecule inhibition of a protein-protein interaction, it seems that DMSO percentage and dilution protocol are important. I...

31 January 2021 6,771 1 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Is it possible to report hydrodynamic loads on different parts of an ocean vehicle while 6DOF motion analysis in Ansys Fluent is running?

It seems a 6dof UDF containing vehicle characteristics such as mass and moments of inertia can be used for specifying a boundary as reprentative of ocean vehicle. It is required to have...

03 February 2021 7,726 3 View

How can I convert netcdf file to a binary file in matlab?

Dear all, I have monthly nutrients data from WOA (in a separate NetCDF file) extracted for a specific region. I would like to convert these data directly into a bin file (one of the separate...

30 January 2021 9,241 2 View

Why and how to avoid negative values in optical density by spectrophotometry when measurements corrected by blank solvent?

I am trying to assess chlorophyll-a content from coral tissue using spectrophotometry (with SPECTROstar Nano, BMG). However, I always seem to obtain some negative values in my optical density raw...

20 January 2021 1,542 4 View

How to get Argo Data? and is this useful for water column SST data?

I am working on MODIS-SST, I need SST in situ data and water column data, Please guide me in this.

21 December 2020 9,935 5 View

Is there a way to automatically gather surface area from a 3D model based on color/texture?

I have been constructing 3D models of large corals and I was wondering if there is a software that I can use to gather the area of live tissue vs dead/disease? I am aware there is a way by tracing...

17 December 2020 6,991 1 View

Robot that helps clean our seas of microplastics?

My team and I are curently working on a project is focused on preventing microplastics and collecting plastic particles up to 2 * 2mm. Our biggest concern is harming sea life and destroying...

29 November 2020 9,240 5 View

Is there a plausible explanation for the increased frequency of severe hurricanes in the southwest Atlantic Ocean other than climate change?

The Southeastern region of the United States of America, including Florida, Georgia, South Carolina, North Carolina, and the Caribbean archipelagos are being devastated by a higher number of...

16 November 2020 9,463 20 View

What are the environmental variables that influence the Northeastern gray whale population?

I am currently working on my M.Sc. thesis studying how climate change affects the presence of the gray whale in one of its main breeding areas in Mexico. We have considered temperature,...

16 November 2020 8,944 3 View

Why do I receive the sae mesage several times?

26 October 2020 409 1 View