How to upload my new research paper?

how to upload my new research paper?

22 February 2021 244 3 View

Is there any research on universal gait and O2 consumption patterns in recreational runners?

15 January 2021 6,479 1 View

How would a 'fair trade' version of a marketplace app like Uber Eats work?

(Note: ResearchGate questions does not have any comments feature. I am unable to directly respond to individual answers below because there is no commenting feature available. Please do not ask...

23 September 2020 5,727 2 View

Which scholars, pedagogues, and projects are carrying forward the legacy of arts educator Elliot Eisner (1933-2014)?

Which scholars, pedagogues, and projects are carrying forward the legacy of arts educator Elliot Eisner (1933-2014)?

13 September 2020 3,664 4 View

Any idea how this image was taken?

A student sent me this image and asked how it was done. My background is in optics, but it has been a number of years since I worked with surface interferometry. I am wondering if you can identify...

12 December 2019 8,423 4 View

Does ANSYS 17.0 workbench have the capability of modelling a fully penetrating - part circumferential defect in a cylinder?

In ANSYS workbench it seems relatively easy to model defects that propagate in a direction that can be described on a Cartesian coordinate system, however, for a fully penetrating - part...

29 July 2018 5,215 3 View

How to determine which Sample Buffers, Running Buffers, and Transfer Buffers to use per Western Blot experiment?

I'd like to be methodical in deciding which buffers to use per Western Blot Experiment. A thorough system of deciding which combination of buffers to use for each WB experiment would be helpful....

17 December 2017 5,382 1 View

How do I go about fashioning a 1 nm wide piece of film and use it to separate two opposite copper plate?

I'm trying to fashion a 1 nm piece of film and use it to separate two opposing pieces of copper plate so they are a nm apart. Any suggestions on how this could be done?

01 February 2016 1,437 2 View

Can anyone help with my Fab antibody expression yield in E coli?

Hi, Do anyone has experience in Fab expression in E coli that ended up with abnormally large pellet (~14g/L, wet weight; and smelly too) which eventually gave low yield (~0.1mg/mL). My Fabs are...

05 January 2016 8,462 4 View

How do I prepare EM grids (negative stain & cryo) without inducing protein aggregation?

I was preparing some negative stain EM grids for protein samples. I purified the proteins with gel filtration right before grid preparation and the traces looked very good (quite symmetric and...

16 November 2015 4,562 11 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

How many of absolute errors (in kcal) do you believe affect the prescription of the diet when estimating the energy required for an elite athlete?

Dear colleagues, I am writing an article that compares the measured and predicted resting metabolic rate in elite athletes. However, to define a cutoff point to determine which equation is more...

16 February 2021 4,515 3 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

SDS-PAGE aggregation problem?

I ran SDS-PAGE with 14% lower gel, 4 % upper gel, having brushing, pre-running empty gel for 30 min. The protein samples had been centrifuged with 14,000g for 10 min, and then the supernatants...

20 January 2021 172 3 View