What is the ideal method in measuring electrolytes and inorganic ions?

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Does the temporal summation of EPSPs accumulate linearly to action potential threshold or logarithmically to a plateau/limit?

I am attempting to determine which current inputs (in uA) are subthreshold for inducing a burst of activity. This is because I am attempting to model DBS form epilepsy in vitro, which clinically...

23 June 2024 4,083 1 View

How to avoid metal contamination from a sonicator?

Hi, I have been using a Q-Sonica Q500 Sonicator to disperse samples on nanocellulose and make emulsions. I have notices black dust like particulates settling out in some samples and on the bottom...

12 June 2024 9,737 6 View

What would be the safety of our roads be with the autonomous vehicles ?

safety of autonomous self-driving vehicles.

26 May 2024 6,880 1 View

What should the phase and pulse duration be for deep-brain stimulation (DBS) for epilepsy?

In DBS settings, I have seen stimulus widths of up to 500 us in duration; however, the most common pulse duration seems to be between 90-150 us. Clinically, mono- or bi-polar electrodes can be...

26 May 2024 5,819 0 View

Why does extracellular cathodic (-ve) stimulation depolarise neurones?

I am a bit confused by these statements: "For extracellular stimulations, a cathodic pulse can generate a current sink in the space immediately around the stimulation electrode, thereby...

11 May 2024 3,318 2 View

Should a biphasic electrical stimulation pulse on neural networks be positive followed by a negative phase or negative followed by positive phase?

I am using a biphasic electrical stimulation on neural networks to attempt to induce electrical kindling for epilepsy on neural networks. In addition, I will be attempting to modulate said...

09 May 2024 7,167 1 View

African philosophy solution to the global problem of poverty?

How can African philosophy solve the worldly problem of poverty?

27 April 2024 6,495 3 View

Is it best to submit literally all of your research plans to your IRB?

Do you submit literally of your research plans to your IRB just to be safe? If not, then what do you use as your cut-off for "submittable" vs "not submittable" given that trainings such as those...

26 April 2024 7,732 3 View

The worldview of indigenous educational values?

Checking the values of indigenous education from different communities in the world

11 April 2024 4,681 2 View

In what ways does the use of LIH tubes impact laboratory workload and efficiency compared to traditional serum tubes?

In the journal, "THE TRANSITION FROM GEL SEPARATORY SERUM TUBES TO LITHIUM HEPARIN GEL TUBES IN THE CLINICAL LABORATORY" by Oğuzhan Zengi, In what ways does the use of LIH tubes impact laboratory...

09 April 2024 7,377 3 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Request Python code?

Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!

08 August 2024 6,242 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Why does everyone use vs code?

Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...

07 August 2024 7,013 4 View

Is an invitation to join the editorial board of Clinical Cardiology Updates a scam?

I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...

06 August 2024 8,981 8 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In the presence of VERY high concentration (1000 ppm) of silicon, the adsorption isotherms of arsenic are showing anomalous trends, why so?

I conducted an adsorption experiment of arsenic on soil in the presence of different doses of silicon as competing ions to see the effect of silicon on arsenic adsorption and desorption. I took 5...

03 August 2024 6,500 3 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View