Why do I get a lot of colonies upon transformation of digested lenti guide puro?

I digested my lenti guide puro plasmid. The only modification in this plasmid is that it has blasticidin resistance gene instead of puromycin. I get the desired bands at ~8kb but I do not see the...

03 August 2020 2,854 4 View

I need images of machine parts (automotive industry) to perform damaged parts detection. Please help!!?

Aim is damaged part detection (i.e. scratch, dent, holes, pits, etc.): e.g. Automotive/Industrial part stamping quality inspection, Rolled sheet metal surface inspection, Pit/hole detection in...

06 May 2018 1,328 1 View

How does degree of intercalation depend on graphite particle size in liquid intercalation (H2SO4-HNO3)? Can oxidation occur in very fine particles?

I wish to intercalate very fine graphite particles through a mixture of concentrated H2SO4 and HNO3. Normally, small particle size results in greater degree of intercalation. Is it possible for...

16 May 2017 5,578 2 View

Calculate value of 'c' parameter for intercalated graphite using XRD data?

01 January 1970 7,752 3 View

How to prepare graphite electrode from graphite powder, to be used in in electrochemical reaction using aqueous and concentrated H2SO4 electrolytes?

01 January 1970 9,238 3 View

How to perform XRD of very small graphite sheet sample?

I have a very small sample of graphite sheet (10mg, 36mm2) of which I need to perform XRD. The detected area of the instrument I am using is 6.25cm2. How can I perform XRD of such small sample?...

01 January 1970 8,838 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View