Why do I get a lot of colonies upon transformation of digested lenti guide puro?

I digested my lenti guide puro plasmid. The only modification in this plasmid is that it has blasticidin resistance gene instead of puromycin. I get the desired bands at ~8kb but I do not see the...

03 August 2020 2,854 4 View

I need images of machine parts (automotive industry) to perform damaged parts detection. Please help!!?

Aim is damaged part detection (i.e. scratch, dent, holes, pits, etc.): e.g. Automotive/Industrial part stamping quality inspection, Rolled sheet metal surface inspection, Pit/hole detection in...

06 May 2018 1,328 1 View

How does degree of intercalation depend on graphite particle size in liquid intercalation (H2SO4-HNO3)? Can oxidation occur in very fine particles?

I wish to intercalate very fine graphite particles through a mixture of concentrated H2SO4 and HNO3. Normally, small particle size results in greater degree of intercalation. Is it possible for...

16 May 2017 5,578 2 View

Will XRD results for intercalated graphite be effected in case of a H2SO4-wetted sample?

01 January 1970 939 3 View

Calculate value of 'c' parameter for intercalated graphite using XRD data?

01 January 1970 7,752 3 View

How to prepare graphite electrode from graphite powder, to be used in in electrochemical reaction using aqueous and concentrated H2SO4 electrolytes?

01 January 1970 9,238 3 View

How to perform XRD of very small graphite sheet sample?

I have a very small sample of graphite sheet (10mg, 36mm2) of which I need to perform XRD. The detected area of the instrument I am using is 6.25cm2. How can I perform XRD of such small sample?...

01 January 1970 8,838 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

How to find the right number of integration points in second order 3d finite elements?

I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...

27 February 2021 6,818 3 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

What is the error of the Txy diagram?

Hi, I know the attached Txy diagram has an error but can't figure it out. Could you please look at the diagrams and explain it to me?

23 February 2021 4,239 1 View

Why the CHi^2 is close to zero with a bad fitting but the Rp and Rwp are still high?

I am refining my XRD data using in FullProf program. The intensity of the modulated X-ray peaks is almost flat. I tried to adjust the peak intensities but unsuccessful. What is the reason? I guess...

23 February 2021 2,304 4 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View