I have all required data (structural data for the phases, XRD input file). I am just unable to locate the option which will allow me to know the percentage composition of the sample.
Thank you so much sir!
Hello all, I am over-expressing acid sphingomyelinase enzyme in HeLa cells. Theenzyme shows expression on western blot but I do not have any activity in in vitro assays. I have checked the...
06 July 2023 365 3 View
Hello, I am using the following tutorial for microRNA profiling: https://drmirdeep.github.io/mirdeep2_tutorial.html I was able to follow it until the patching step which is: bash...
31 October 2022 6,779 0 View
Does anyone have experience growing single cell clones of MCF-10A.I have generated gene knockouts and need to pick single cell clones from these cell lines. It is challenging as these cells are...
28 April 2021 1,857 2 View
I am using MCF10A cell lines. I see different sensitivity to Blasticidin of my control cells (not infected with lentivirus) vs cells infected with lentivirus. I thought this is a good thing as My...
25 February 2021 7,066 1 View
I am making CRISPR knockouts in MCF10A cells and Blasticidin is used as a selection marker. When I add Blasticidin, some cells die which is expected as they haven't received my sgRNA. However,...
11 December 2020 4,710 2 View
I digested my lenti guide puro plasmid. The only modification in this plasmid is that it has blasticidin resistance gene instead of puromycin. I get the desired bands at ~8kb but I do not see the...
03 August 2020 3,001 4 View
I have added anti- CD28/anti-CD3 beads to in vitro culture of murine T cells. Some of the cells have increased in size which indicates their activation. However, is there a method to quantify the...
08 August 2019 3,538 7 View
Aim is damaged part detection (i.e. scratch, dent, holes, pits, etc.): e.g. Automotive/Industrial part stamping quality inspection, Rolled sheet metal surface inspection, Pit/hole detection in...
06 May 2018 1,401 1 View
I wish to intercalate very fine graphite particles through a mixture of concentrated H2SO4 and HNO3. Normally, small particle size results in greater degree of intercalation. Is it possible for...
16 May 2017 5,703 2 View
Methane cracks to hydrogen and carbon. moles of hydrogen and methane are known.
23 June 2016 4,817 5 View
I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...
07 August 2024 5,177 3 View
Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...
03 August 2024 7,843 1 View
If you think of electrons with spin as bar magnets, you know bar magnets of opposite polarity as long as they're not occupying the same spatial location don't cancel out each other's magnetic...
03 August 2024 3,927 2 View
I am in search of a modified Hummer's method, which can be used to synthesis graphene oxide within 6-8 hours. As I am a student it is not allowed for us to work after 5 in the laboratory. So I am...
01 August 2024 8,368 2 View
Google scholar isn't giving me any results on this
27 July 2024 595 2 View
I want to know more about ore deposits in world.
27 July 2024 3,827 4 View
The catalytic effect of retained austenite refers to the phenomenon wherein quenching steels with a small amount of retained austenite, when subjected to tempering at a specific temperature...
27 July 2024 8,508 0 View
Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM or SEM images?
25 July 2024 5,482 2 View
Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...
25 July 2024 2,309 5 View
I would like to know the role of the TFA (trifluoroacetic acid) used on reverse phase chromatography c-18 column. It's is often used with a concentration of 0.1% on the mobile phase.
23 July 2024 8,075 3 View