This is an easy-to-use 10% (w/v) solution of purified Triton™ X-100 detergent for use in routine and high-demand protein research methods and molecular biology techniques. Triton X-100 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or other solutions intended to extract and solubilize proteins. Lysis of  cultures of Streptococcus faecalis ATCC 9790  is made possible by providing exposure to both anionic (sodium dodecyl sulfate) and nonionic (Triton X-100) surfactants.Triton X-100 may induce cellular lysis by releasing a lipid inhibitor of the cellular autolytic enzyme

Yes you can use. I used Triton-X 100 (1%) against P. aeruginosa as positive control for Sytox green. It give good fluorescence