As I need to isolate RNA from FFPE blocks using kit, i need to know the valuable answers before I start with the procedure. Pls share your experience if any
Deparaffinization is the same as for DNA isolation. Since formaline results in degradation of DNA/RNA, I recommend to use the shortest random primers (oligo 6 - 8) for cDNA synthesis. As for RIn, it depends on the quality of tissue in block. Ask your cytologists to identify the site free of necrosis and connective tissue within the block and use this exact piece for the extraction of RNA.