We want to measure the protein inhibitor of the enzymes used.i need to know after enzyme immobilization graphene quantum dot will be completely turn off?
I guess it is dependent on the surface coverage of enzyme. If enzyme you used is much larger than GQDs (small one, 3-5 nm), it would be completely turned off. How did you assemble enzyme-GQD conjugates?
thank you Professor and sorry for delay.... after synthesis of graphene quantum dot , i add enzyme in it and wait I wait to be immobilized... enzyme will be immobilized by covalent bonding of carboxylic group of quantum dot and amine group of enzyme.this is my idea