Wiht Percent signal change?

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How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

What happen if a transcription factor bind directly to not only the regulatory region but also the target gene sequence?

As the title described, it is common that a target gene sequence contains motif that acts as the binding site of transcription factor rather then the upstream regulatory region. Does this affect...

13 May 2024 1,099 3 View

Antibody maleimide conjugation - TCEP method affected by tris concentration in purified buffer?

As title describes, we're trying to mutate an amino acid of antibody Fc to cysteine, followed by TCEP to further drug conjugation, one of my colleague had raise the concern of purified TCEP...

29 April 2024 3,227 2 View

Can I use a different grade of cells for my positive control?

I am performing PCR as a QC test to look for a transcription gene that should be negative after a CAR T therapy process. As we are comparing against a CAR transduced patient's cell, we require a...

22 April 2024 5,262 1 View

Which software is better for simulating superconducting magnetic fields?

If I want numerical simulation software to simulate superconducting magnetic shielding, which software is better for simulating superconducting magnetic fields?

25 March 2024 5,589 1 View

How do you remove snow particles on cryoboxes that were stored in LN2?

I have difficulty on removing snow particles on cryoboxes, storing cryovials. The snow particles can sometimes take a lot of space and causing difficulty in closing the cryobox which will later be...

19 February 2024 6,690 0 View

What is the practical value of the 'scale' parameter in Hspice '.trannoise' command?

HSpice transient noise simulation command '.trannoise' has the following parameters: .TRANNOISE output [METHOD=MC] [SEED=val] [START=val] + [FMIN=val] [FMAX=val] [SCALE=val] +...

18 January 2024 3,193 0 View

How to avoid false positive of gene delete by PCR in Yarrowia lipolytica?

Hi all, I have been trying to knock out genes in Yarrowia lipolytica using CRISPRyl system, but it always appears right for initial verification by colony PCR (PCR products shorten as up/down...

17 January 2024 5,089 0 View

What are the future development technologies for microbial communities in the ecological environment?

microbial communities

11 January 2024 8,106 0 View

Is there any proper method to make Cross-sectional specimen of Alkali metal electrodes in lab??

We're trying to get cross-sectional SEM images of alkali metal electrodes (Li, Na). we cut by our lab-knife or lab-scissor as neatly as possible, but results were unsatisfied. Is there any...

03 January 2024 7,140 2 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

31 July 2024 8,666 3 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

How can I extract my bibliography from researchgate ?

how can I extract my bibliography from researchgate ?

28 July 2024 6,737 1 View

How can biogenic synthesis techniques be applied to develop plants capable of extracting and processing heavy metals from contaminated soil, and wha?

Understand how to utilize biosynthesis to develop natural and sustainable solutions to address heavy metal pollution and improve environmental and agricultural conditions.

26 July 2024 1,469 1 View