I am going to sacriface my animals in a couple of weeks and dissect plenty of tissues in a day. There is no way to do all protein and RNA isolations at the same day. Therefore I must freeze (-80C) some tissues for at least a few weeks and isolate part by part.

Could you please enlighten me about RNA-later solution (invitrogen-thermofisher)? Can I put my tissues into RNA-later solution directly or required to homogenise my tissues in RNA-later solution before freeze it?

Also I am quite concern about protein extraction from tissues. Can I extract proteins from frozen tissues and if it is possible what kind of solution require to freeze tissues? Or freeze it without adding any solutions?

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