Hello,
I am developing a method for some derivatized metabolites using 3-NPH (nitrophenylhydrazine). I did the compound optimization for the derivatized compounds individually, but when I started injecting these compounds (individually) on LC, I noticed multiple peaks in the chromatograms of a couple of compounds. I added 5 MRMs to each compound in the LC-MS method to check which one is the major in all MRMs for the same compound, but I noticed that some MRMs are giving peaks at different retention times from other MRMs (all for the same compound). Is there anyone who experienced such a situation before? What would you do to resolve this issue and understand what is happening? I could not find much literature using the metabolites I am interested in using the same derivatizing agent.
I hope I find some advice or ideas to figure this out!
I appreciate any helpful answers :)
- Badges
- Science topic
- Similar topics
- Microbiology
- Assays