The role of tween 80 solutions with a variety of concentrations (surfactant) is for solubility purposes. In principle, you can replace it with ethanol or DMSO, however, you should be careful not to use large quantities of DMSO or ethyl alcohol to avoid any toxicity.
The following link contains a study in which ethyl alcohol and tween 80 were separately used and the results in both media were similar (see methods section):
http://www.hindawi.com/journals/ecam/2011/237941/
The following links contain studies where tween 80 was used:
The role of tween 80 solutions with a variety of concentrations (surfactant) is for solubility purposes. In principle, you can replace it with ethanol or DMSO, however, you should be careful not to use large quantities of DMSO or ethyl alcohol to avoid any toxicity.
The following link contains a study in which ethyl alcohol and tween 80 were separately used and the results in both media were similar (see methods section):
http://www.hindawi.com/journals/ecam/2011/237941/
The following links contain studies where tween 80 was used:
Actually, DMSO and Tween 80 are just act to increase solubility like a surfactant.
During animal model pharmacological screening, we dissolve all the crude extract in water. So, here as you know that all the fraction you got from your partition is not water soluble except the Water soluble fraction.
So, to deliver the complete dose in your desire concentration you need to solubilize that extract completely.
Normal. what i did is that when i Did "brine shrimp lethality assay" i used DMSO but when i checked the pharmacological activity in mice model then i used Tween 80. The basic different is the toxicity profile of these solvents.
But Whatever you use DMSO or Tween 80, always use these also with your control samples. then will get a clear understanding avoiding the dominance of these Tween and DMSO.