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Questions related from Sikder Nahidul Islam Rabbi
In every leaflet of restriction enzyme we find a booklet about the reaction composition, incubation temperature and reaction guidelines for every specific restriction enzyme. Here, I have...
12 November 2016 9,070 11 View
Here, I have done a pcr and got pcr product about 470 base pairs. Now, I want to do a second pcr from that 470 bp PCR product (using it as template DNA). So, how can I recovery that pcr (470 bp)...
08 March 2016 5,704 23 View
We are using carrier RNA during extraction procedure of HBV DNA, according to our commercial kit protocol they said to use it. We are using it with the samples and others pre-mixed reagent during...
12 November 2015 3,520 6 View
In my lab we normally do cell culture (For karyotyping) from fresh sample, just after collecting in heparinized tubes. Now we are thinking of doing some sample which will come from a longer...
27 July 2015 1,481 15 View
We all know that maximum matching in HLA typing is important in organ donation process, higher the matching lower the chance of organ rejection related issues. As HLA protein (gene located in...
16 July 2015 4,894 33 View
Here I am using two different wavelengths to target different moiety in BSA by fluorescence quenching method. Where I kept BSA as fluorescence active and drug (fluorescence inactive). Now I am...
17 March 2015 2,042 5 View
I used to PCR-PFLP,but considering the cost of restriction enzyme and time consumption, i am thinking to study SNPs by SSP-PCR method.Is it possible to study SNP or several SNPs at a time ?then,Do...
11 January 2015 9,944 3 View
I want to keep WBCs (usually 2 to 3 days after collection). After separation, cells were kept by mixing with RPMI, then the mixture was kept at 4°C. After one day, all cells were dead. Normally, I...
09 December 2014 501 9 View
We all know that cancer is mostly caused by rare genetic mutations of a specific gene. We also know that DNA polymerase III repairs mismatches of base pairs during DNA replication or recombination...
01 December 2014 3,286 4 View
In my lab I use Olerup-SSP HLA-Typing tray kit for HLA-A,B,DR typing of patient and donor prior to check the match-ability for organ transplant .We use manual (data sheet) to find the serology and...
22 November 2014 3,772 2 View
