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Questions related from Sikder Nahidul Islam Rabbi
In every leaflet of restriction enzyme we find a booklet about the reaction composition, incubation temperature and reaction guidelines for every specific restriction enzyme. Here, I have...
12 November 2016 9,018 11 View
Here, I have done a pcr and got pcr product about 470 base pairs. Now, I want to do a second pcr from that 470 bp PCR product (using it as template DNA). So, how can I recovery that pcr (470 bp)...
08 March 2016 5,638 23 View
We are using carrier RNA during extraction procedure of HBV DNA, according to our commercial kit protocol they said to use it. We are using it with the samples and others pre-mixed reagent during...
12 November 2015 3,447 6 View
In my lab we normally do cell culture (For karyotyping) from fresh sample, just after collecting in heparinized tubes. Now we are thinking of doing some sample which will come from a longer...
27 July 2015 1,395 15 View
We all know that maximum matching in HLA typing is important in organ donation process, higher the matching lower the chance of organ rejection related issues. As HLA protein (gene located in...
16 July 2015 4,837 33 View
Here I am using two different wavelengths to target different moiety in BSA by fluorescence quenching method. Where I kept BSA as fluorescence active and drug (fluorescence inactive). Now I am...
17 March 2015 1,985 5 View
I used to PCR-PFLP,but considering the cost of restriction enzyme and time consumption, i am thinking to study SNPs by SSP-PCR method.Is it possible to study SNP or several SNPs at a time ?then,Do...
11 January 2015 9,881 3 View
We all know that cancer is mostly caused by rare genetic mutations of a specific gene. We also know that DNA polymerase III repairs mismatches of base pairs during DNA replication or recombination...
01 December 2014 3,228 4 View
