What will happen if someone to be in darkness for 10 years?

Physiological changes

08 September 2014 629 1 View

How to clone and express the desired gene in plants using Agrobacterium rhizogenesis?

I am planning to express and produce the desired protein in hairy root culture. Can anyone help me in the selection of vector and other requirements?

01 February 2014 6,408 3 View

How does the secondary metabolic status change in protoplast fusion plants?

I am curious to know about the secondary metabolism of protoplast fusion plants (Different species Ex. One plant from Fabaceae and another one from Asteraceae). Is it possible to do this and how...

01 February 2014 4,475 9 View

What is the best way to approach PDF in Europe?

Post Doctoral Fellowship

31 December 2013 6,659 4 View

What are the elicitors used in the enhancement of flavonoid in callus culture?

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11 December 2013 1,547 3 View

How to prepare preparative TLC plates?

I have prepared TLC plates using Silica gel G (Merk). I could not even get the surface. Can anyone help me?

10 November 2013 9,622 10 View

Can anyone tell me about the recent development in chromatography techniques?

i.e. Isolation of metabolites from natural sources.

10 November 2013 6,969 0 View

How does one prepare the crystals from plant extracts?

Can anyone tell me about the crystal growth procedure from plant extracts?

09 October 2013 4,557 10 View

Can anyone tell me about RNAi in plants?

I am planning to do RNAi in plants. What are the criteria I should consider before starting my work?

09 October 2013 4,326 4 View

What are the merits and demerits of green synthesis and chemical synthesis of nanoparticles?

Most of the articles (Green synthesis) mention that the functional groups were reduced and attached with the nanoparticles (FTIR analysis). Is it better delivery of a drug than nanocoating?

08 September 2013 9,965 16 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View