Preparative TLC is used for analytical separations of larger quantities of materials. Because sample mass loading capacity is proportional to the square root of the sorbent layer thickness, thicker layers are used (>250μm up to 1 mm). When a binder is required, the softer inorganic binders (like calcium sulphate) are used so the sample bands can be easily removed. The compounds to be separated are often applied as long streaks, developed and then recovered by scraping the adsorbent from the plate and eluting with a strong solvent.
hi Dear sir, first take some amount of silica power in a beaker and add some amount of water and star well 15 mint and add water for needed and make salary type past and this salary or past fallow on TLC plate before arrange plate slop type fallow of past top of the plate some amount and give vibrate to plate till equivalent thickness of past on whole plate and dry to this TLC plate at 105C in oven for 1hour. and now use of this TLC plate for separation technique.