Sometimes while culturing the U87 cells, we get this cluster growth. Can anyone explain the reason?
U87 cell line does not tend to aggregate like this....
You have to rule out the possibility of contamination with other cell line, or the Mycoplasma infection, and the serum quality in the culture medium.
Short Tandem Repeat (STR) test provided by Promega is useful to know the cultured cells are really U87.
Can anyone plz tell me why it is dendritic like growth in U87 cells, actually one day earlier the cells were good but next day entire flask was filled with cells and media was yellow and also the numerous crystal like structures. Please help
Can anyone plz tell me why it is dendritic like growth in U87 cells, actually one day earlier the cells were good but next day entire flask was filled with cells and media was yellow and also the numerous crystal like structures. Please help
Can anyone plz tell me why it is dendritic like growth in U87 cells, actually one day earlier the cells were good but next day entire flask was filled with cells and media was yellow and also the numerous crystal like structures. Please help
I used to have this problem with the U87MG the way that I resolve it it is that I do not allow the cells to grow over 5x10^6 per flask. If you grow the cells over 10x10^6 it will start to form some clusters, however you can pippette them with a 1000 tip up and down several time to destroy the clusters. If the cells continue to do this clusters the cells might have undergo some stress and they will not recover
Hope it helped
Sometimes, U87MG cells can form that kind of aggregates.
Usually this behavior appears at high density and with low amount of serum in the medium.
There are a couple of publications scoring this phenomenon:
1) Ledur et al. 2011, Extracellular ATP reduces tumor sphere growth and cancer stem cell population in glioblastoma cells
2) Zhou et al. 2011, Metabolic alterations in highly tumorigenic glioblastoma cells
So, Shabana, if you are not interested in this "3D aspect", just thaw a fresh aliquot of cells and do not let them reach a high density while growing.
Shabana Usman Simjee Actually if the cell is too many in culture dish, they tend to form spheroid like formation. But usually it happen on the edge of culture dish. Try to culture the cell with lower number it will help the cell not to form the spheroid like formation.
Also if you culture the U-87MG for too long time like 4-7 days without do subculture, they also tend to make this kind of formation. That's what I know
Normally U87MG forms these clusters when they are at higher density. You can remove these clusters from cultivation and have a more homogenous population. For example during passaging, you have your cells in suspension including clusters. However clusters or spheroids are much heavier, so you can mix your suspension and wait for few seconds in order to let them sink faster than cells. Consequently, you use only supernatant (containing cells, not clusters) for new dishes. Anyway careful with higher densities of these cells. Good luck :).
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