Hello everyone, I have tried to correlate the results of the following analyzes: (total polyphenols, total flavonoids, condensed tannins and flavonols) with the IC50 of the DPPH antioxidant activity test. can you please help me interpret these results? I don't understand why the correlation is weak for polyphenols and flavonoids. My samples are the leaves and stems of a plant extracted by 2 different extraction methods L: Leaf S: rod L1: Leaf (2nd method) S1: Stem (2nd method)
Check https://www.tandfonline.com/doi/pdf/10.1080/13880200601113131
Also check https://aip.scitation.org/doi/pdf/10.1063/1.4985410
The antioxidant activity may mean the rate of a given molecule with a radical or the total amount of antioxidants in your samples. The DPPH test measures a mixture of both. Total amount of phenolics or whatever is measured by a protocol based on certain chemistry behind the method. Therefore, I would not expect that any correlation exists.
If you look your R2 factor for all compounds, can be concluded that your antioxidant activity originates from flavonols. The flavonols, called 3-hydroxylavones, are the most widespread of the flavonoids in plants, which is strange, because your correlation between flavonoids and antioxidant activity is very small. Flavonols offer powerful antioxidative effects due to their structure, the additional presence of hydroxyl groups and double bond conjugated with a keto function. It may be best to double-check the correlations you received.
It would be good to do LC-MS analysis of your extracts to see which compounds are most present in the extract, and responsible for the antioxidant activity.
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