How to predict traffic during vehicle routing using SUMO and Traci?

Dear Experts, I have executed Ant colony algorithm,which gives optimal route in terms of trail of junction of node. ACO considered parameter of Distance and Pheromone. Now how to update this...

16 December 2019 5,245 3 View

What will be the possible Interaction properties Between the two layers of 3D printed part in ABAQUS ?

FEA on 3D printed Part for Analyze its mechanical properties.

09 November 2019 6,331 2 View

How to model slab section for pushover analysis in SAP2000 ?

I am performing pushover analysis on a RCC building in SAP2000. Whenevr I model Slabs as thin-shells, beams connected to them exhibit a brittle behaviour and fail directly after the yield point....

29 April 2019 4,162 5 View

Is proton NMR is same or different of a polymer after curing or before curing?

Cured and normal polymer have different Proton NMR or not.

24 February 2018 9,517 4 View

Any resources for understanding the Shishkin Mesh and the HODIE scheme?

I want to implement the HODIE scheme on a shiskin mesh for singularly pertubed reaction diffusion equations.

30 May 2016 3,572 1 View

Anyone know of a dictionary based string matching algorithm for python?

09 March 2016 2,766 4 View

How to perform gromacs MD simulations of a protein to get the atomistic details of its unfolding at different temperatures?

I want to perform a MD simulation of a protein at different temperature to check its stability and unfolding at different temperature (300K, 310K, 320K etc.).

29 June 2014 5,632 4 View

How to increase the efficiency of transferring large molecular weight proteins ~265 kDa for western blot analysis?

I am trying to analyse acetyl CoA carboxylase (ACC) protein (~265 kDa) using Biorad Criterion gels and Nitrocellulose membranes. I have already tried separating it on a 7.5% gel with 4% stacking...

16 June 2013 6,282 7 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

Good day, I have not been able to amplify the plasmid Frt-hspB1. Somebody could give an advice me?

I acquired the plasmid in Addgene, reconstituted the filter paper where the plasmid comes from with 30 microliters of TE buffer pH 8, immediately afterwards perform the transformation of E.coli...

25 January 2021 2,526 3 View

Has someone used TTC agar overlay method and can help me?

I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...

22 January 2021 585 10 View

I am having trouble with cloning my gene. How can I fix this?

16 January 2021 6,566 8 View