sorry i dont know

Hi Carlos , 

Thats Ok Not A Prob.

Sperm produced in the testes are not completely matured, and they pass through epydidymides for further 10-15 days (or more) to complete the maturation process. During this migration, various proteins and molecules are added to different parts of the sperm (plasma membrane, Acrosome region etc.) and activate different functions and cell signaling pathways. In the tail region, proteins cross-linked by disulfide bonds and stiffen its structure to attain motility. This maturation process is not completed of sperm in caput and corpus region of the epididymis. Hence, the motility or fertilization capacity may vary in sperm aspirated from different regions of the epididymis.   

Sperm motility can slightly enhance by pentoxyfilline. Some authors smuggest that co-culture with epididymides cells can improve the sperm motility.

Hi Aruna , 

I agree With ur answer but , i have observed there is no fertilization decrease in the pesa sample compared to normal ejaculate , but as u said if complete maturity is not attained when the sperm is in the eppidymis and at that particular time if its been ejaculated normally i still think motility will sustain more ... in the ejaculated sample !!! compared to PESA sample

Do you think seminal plasma play any role in motility  as pesa sample will be lacking that ?

 Thanks & Regards

Aamir Javed  

Hi Aamir

You are extremely correct. Accessory sex glands (specifically prostatic secretions) contain numerous factors which help to enhance motility, zona binding and natural fertilization capacity of sperm. 

fertilization capacity of PESA sperm remains high as they have already complete the DNA packaging. 

best regards

Hi Aamir

Seminal plasma is essential and very important to the sperms motility and it contains the essential elements for sperm nourishment that provide sufficient energy to the sperm motility. In addition to that the acrosome reaction of PESA sperm may be not effected, this is why the fertilization capacity of PESA sperm remains high. Acrosome reaction  is not related to sperm motility , you can find high motility% in some semin samples with low fertilization rate due to low acrosome reaction% and you can find some semin samples with 60% or less motility rate with high (85% or more ) fertilization rate. Check the acrosome reaction% in both PESA sperm sample and  ejaculated sperm sample. 

Best regards

Ali

Hi Ali and Aruna,

So Do you think that addition of the seminal plasma to the PESA sample will prolong or enhance the motility ? 

Regards

AJ

I think better to try and see and compare between tow samples with and without seminal plasma. make sure the seminal plasma from the same subject.

good luck

Ali

Hi Aamir

Your hypothesis is partly true. But it depends on the state of maturity of sperm. If your sample is from lower caput region the sperm are more immature. Other thing is seminal plasma of some subfertile males are deffective and using the seminal plasma from same male may not produce encouraging results. Better to try with seminal plasma from both patient and fertile individuals. My suggestion is only for research purposes. You need to go long way for clinical practices.

The sperm has not been matured enough.

Thanks Javad

Dear Ali And Anura ,

Thanks For Ur Valuable Suggestion , I will Try and See whether it improves or not the motility and let u know .

Regards

Aamir Javed 

Good luck

welcome and good luck