Hello Mr. Bastola,

https://media.cellsignal.com/pdf/5633.pdf

Late of the second paragraph, it says " SB203580 inhibits p38 catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 by upstream kinases. "

Hello Mr. Bastola,

https://media.cellsignal.com/pdf/5633.pdf

Late of the second paragraph, it says " SB203580 inhibits p38 catalytic activity by binding to the ATP binding pocket, but does not inhibit phosphorylation of p38 by upstream kinases. "

to test whether the inhibitor worked you need to probe for phospho-MAPKAPK2, which is often next in line in the signaling cascade.

Thank you so much Mr. Akar and Mr. Rosenzweig !!

Hello Akar,

Yes, the inhibitor inhibits the activation of MAPKAPK-2 by p38 MAPK.