How Artificial Intelligence and Machine Learning are helping to find COVID-19 drug discovery?

How are Artificial intelligence and Machine Learning boosting COVID-19 drug discovery process?

31 May 2020 2,570 6 View

How Artificial Intelligence/Machine Learning/ Data Science can help to eradicate poverty?

Can AI/ML/DS really help to fight poverty?

21 May 2020 4,694 19 View

Where we can obtain few benchmark datasets for high utility mining?

http://fimi.uantwerpen.be/ Apart from this, suggest few benchmark datasets for high utility mining.

18 May 2020 3,846 1 View

What are the countries doing pool testing? Any statistics available regarding pool testing...?

Any website or research that gives details of pool testing data

17 May 2020 512 4 View

How governments using Data Science/Artificial Intelligence to fight coronavirus?

1. What are the countries using Data Science/Artificial Intelligence to fight the pandemic? 2. What are the Data Science methods used?

14 May 2020 5,449 15 View

Any one interested in publishing papers in fluid dynamics?

willing to publish a paper

03 June 2019 5,402 2 View

Can anyone suggest about rearing techniques of diamond back moth (Plutella xylostella) pest?

Rearing techniques for the varieties of plant associated insect pest especially Diamondback moth.

17 February 2019 1,783 2 View

Mutiple shoot induction in Banana?

I'm working on micro propagation of banana. can any one suggest me the exact protocol for induction multiple shoot from young suckers of banana.

28 December 2018 8,317 3 View

How to prepare an effective research proposal in social sciences?

I want to prepare a research proposal where, i want to include the historical heritage and tourism potential of area of my study to generate more employment opportunities in that area.

18 March 2018 4,292 4 View

No title

31 October 2017 449 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View