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Questions related from Beatriz Botasso Gomes
The 260/280 ratio is ok between 1.8 and 2.0 however we were unable to advance due to the 260/230 ratio
14 May 2024 7,503 3 View
We already used milk: 5% to 1 hour, 7% to 2 hour, 10% to 1h, 5% overnight, but, , in all blockages it still marks the entire membrane, every run, there is a large band with no specific mark HELP
21 February 2024 7,304 0 View
I'm going to use the RIPA buffer, but I don't know if I should use the sonicator or polytron homogenizer
31 January 2024 7,385 1 View
I am trying to do a western blot of cytokines (iL-10, IL-6) of muscle tissue, using a strip of lysis buffer, 50 ug of proteins and blocking in 5% skimmed milk, however no signal appears, any tips?
25 January 2024 6,985 2 View
I need to quantify microglia and astrocytes through immunofluorescence, however I would like to know the best way to quantify, whether fluorescence intensity or manual counting or some other type
20 November 2023 167 3 View
