Hi all,
My lab technician has a simple question about solution II preparation. He prepared solution II for plasmid prep so many times, everything are OK, but after we moved to new building, he had a trouble in solution II prep, he firstly dissolved 8 g NAOH in ~800 ml DiH20, heated the solution with heat stir (VWR), after the NAOH completely dissolved, then added 10 g SDS (also tried 100ml of 10% SDS)， the solution immidately became turbid, SDS couldn't completely dissolve after a couple of hour ,why? Thanks a lot for your help in advance.
Doesnt the preciptate redissolve when you warm the solution again ? The same happens with my prepration on cold days but the preciptate redissolves when I leave it in the 65oC hot air oven for 5-10 mins.
- Prepare 1% SDS stock solution first and then add NaOH.
- You can prepare two stock solution separately and mix before use.. as given in protocol below:
http://2015.igem.org/wiki/images/3/30/ITB_INDONESIA_IGEM2015_DNA_Plasmid_isolation.pdf
3/31/21
Dear Aiguo,
I don't know if this will help. It seems that you never had this problem before, but noticed it when you moved to another building. Have you checked the quality of the water in the new building? Does the building have a functioning water softener that needs re-charging? The water may contain low levels of calcium that cause the SDS to precipitate. ???
I hope this information is helpful.
Bill Colonna Ames, Iowa, USA [email protected]
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