Aiguo Xu

5 Questions 22 Answers 0 Followers

Questions related from Aiguo Xu

How to clone gene including Poly A into vector?

Hi All, I tried to clone 12 gene, about 1kb, including ploy A (40) into pUC57 individually, using NEB stable cells, but only 2/12 have successful clones, the other 10 can't get the correct clones,...

07 May 2021 6,979 0 View

Why SDS didn't dissolve well in Solution II?

Hi all, My lab technician has a simple question about solution II preparation. He prepared solution II for plasmid prep so many times, everything are OK, but after we moved to new building, he had...

31 March 2021 1,695 4 View

Do you have experience of second round PCR amplification issue?

When 1st-round PCR amplification was finished , I could get a clear expected band, but the amount was very low, so I cut the band and run second-round PCR with same condition, it didn't work, I...

22 January 2021 1,138 4 View

There are so many COVID-19 detection kit in the markets, it could detect N, E and ORF1ab, which one is reliable for patient dignostics?

I am confused with so many kits, need to know which one is the best?

31 March 2020 7,397 6 View

Filter paper for plasmid prep

When I prepared plasmid, after added solution 3, centrifuged the lysate at 10,000g, 10 min, before pour the supernatant into column, I still can see some floating debris in the supernatant, how I...

01 January 1970 8,345 3 View