To be developed.
I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...
22 February 2021 6,937 3 View
Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....
14 January 2021 6,326 2 View
I am working with Balb/c mice, and in my experiment, I have to inject mice with antigens, then get their spleen and collect the splenocytes for fusion. Recently, I get into trouble with the mouse...
04 January 2021 3,591 3 View
I am looking for articles, coferences, publications or thesis concerning the capture of solid oleophilic polymers in solution in water. Thanks Thierry Le Blan
01 January 2021 5,798 3 View
Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...
27 December 2020 3,688 3 View
Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...
01 December 2020 1,178 3 View
Dear all, I want to choose a normal ovary cell line and check for its proliferation property when introducing carcinogenic factors. Does anyone have any suggestions for the cell line? Many thanks...
08 November 2020 9,100 3 View
Dear all, I find a paper using Snapback primer. I do not understand the function of snapback tail? What is snapback primer? When do we use snapback primer? and why we use that? Thank you.
01 November 2020 8,869 2 View
22 October 2020 9,354 4 View
I am planning to characterise the transcriptome of pre-anthesis kiwifruit anthers. The samples are being harvested on my behalf and sent down by post, and I cannot ask the harvesters to dissect...
10 October 2020 1,830 3 View
I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...
01 March 2021 3,610 1 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...
01 March 2021 9,511 3 View
We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...
23 February 2021 1,898 1 View
Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...
21 February 2021 5,143 2 View
The sections are about awareness, practice, and perception regarding waste management. Thanks
20 February 2021 5,722 3 View
I am after quantifying the blue stained collagen in a Masson's-Trichrome stained skin section from an immune deficient mice and compared the same with its WT control. I am curious to know can...
17 February 2021 384 3 View
Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...
14 February 2021 4,959 1 View
i am doing research on E.feacalis that is grown in dentin and after that, we did a gram stain for the sample my Q first does the gram stain consider as reliable tool for testing and seeing the...
14 February 2021 6,697 1 View
Hi everyone, I am conducting a non-linear multi-layer pavement structure analysis which is a stress-dependent. So far, I am able to define the nonlinearity for one soil layer for its resilient...
11 February 2021 9,607 3 View