Just a quick question above, this oxidation method is mentioned by literature, but no one talked about the principle. Can anyone figure out how the oxidation works on any specific group?
I don't want to be 'indoctrinating you' anyway....
and - unfortunately - I am no chemist or biochemist - and I am out of 'business' for 5 years now (retired)....
Since (worst case) nobody seems to be willing (or has the time now) to give an answer or (that condition would sound a bit better) - has found your question within the 7 days that passed by now I would like to reply to your Question (but - I regret - it is not a 'mathematical equation...short and precise...), instead some work, I guess:
If you had a look into:
Article Effect of peracetic acid on the enzymatic digestion of vario...
you may have an imagination that the considerations / thoughts on such staining problems , recte: 'staining reaction mechanisms' were thought long long ago...
NB: Direct LINK to that paper (omit the 2 underscore characters ' __ ' inserted here when copying and pasting into your browser:
"EFFECT OF PERACETIC ACID ON THE ENZYMATIC DIGESTION OF VARIOUS MUCOPOLYSACCHARIDES: REVERSAL OF THE PAS STAINING REACTION OF MUCIN"
FULLMER HM, 1960:
"....The-oxidation of sections with peracetic-acid profoundly modifies the effect of both proteolytic acid mucopolysaccharase enzymes on mucins and other mucopolysaccharides as well as inducing the staining reactivity of several mucopolysaccharide-containing structures with aldehyde fuchsin....."
Another article by the same author:
FULLMER HM, also 1960:
"A COMPARATIVE HISTOCHEMICAL STUDY OF ELASTIC, PRE-ELASTIC AND OXYTALAN CONNECTIVE TISSUE FIBERS"
Article A comparative histochemical study of elastic, pre-elastic an...
(for copying and pasting the following URL into your BROWSER the same as above applies:
but there - for sure - are more "modern" studies explaining what is known and what is not... , e.g. (naturally not exhausting...searching PubMed, Google scholar, Google and other data bases for the respective keywords will show many other articles too):
LEONARDI et al, 2001
"Histochemical study of the elastic fibers in pathologic human temporomandibular joint discs" in: