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Questions related from Shan Lu
Recently I noticed that air bubbles constantly appeared from my pump and keep clogging my columns. 【Solvents】 Solvent A =sodium acetate buffer (0.05 M, pH 6.0) in water containing 5 mM β-CD...
12 April 2022 5,019 5 View
Hi, I am studying HPLC quantitative analysis. For the standard addition method, based on my study, I need to make a calibration curve for each sample. For each curve, I need to prepare several...
23 October 2021 4,883 5 View
Hi, Everyone I have a statistical issue. I divided all my samples into type and age groups: 1. Young A; 2. Old A; 3. Young B; 4. Old B. I need to compare A vs B under young and aged groups,...
30 September 2021 3,910 3 View
Just a quick question above, this oxidation method is mentioned by literature, but no one talked about the principle. Can anyone figure out how the oxidation works on any specific group?
21 December 2020 6,002 1 View
Before cryosection, tissues can be simply fixed (4% Paraformaldehyde and then sucrose dehydration). In theory, any tissue fixed by paraformaldehyde should go through an antigen-retrieval...
09 November 2020 9,435 4 View
I have an old HPLC machine. Every time when I put my filter into 50%:50% water and acetonitrile mixture, my filter always generate bubbles. However, once I change the solvent to 100% acetonitrile,...
09 March 2020 5,821 4 View
