Hello every one. I need help please!
I did transient expression in Nicotiana benthamiana using GV3101 Agrobactirum strain. The bacterial cultures which have GFP were co-infiltrated with equal amounts of an Agrobacterium suspension of a p19. After 5 days the plants were analysed by confocal microscopy. Unfortunately I couldn't find anything and there was no signal for GFP.
I did this two times now and it did not work at all.
Can anyone please give me any suggestion that might help.
Thank you
firs thing goes first, are you sure that you have get the right vector in GV3101?
second, have you tried collect the plants after two days, since it was transient expressed.so perhaps those genes you transferred had been silenced by the p19.
Hi thanks for your answer. Yes I am sure about the vector. But I screened them after 5 days some of our lab members told me that GV3101 is usually screend after 5 days. Do you think I have to screen them in 2 days?
- Badges
- Science topic
- Similar topics
- Microbiology
- Assays