why my pseudovirus construct is not showing fluosrence in hek ace2 cell line
Please investigate the effect of temperature and strain rate of hot deformation and the formation of delta ferrite in martensitic stainless steel 410?
25 July 2024 9,408 0 View
Hi, my research involves in ZnO synthesis. I have performed rietveld refinement of my samples and obtain a good fit using Fullprof suite software. However to obtain the crystallite size, the IRF...
07 July 2024 3,526 3 View
I need to put an extra motor on the knee to do part of my project. The process of doing it is needed in the use of other software and sub-programs.
03 July 2024 6,038 0 View
we need surface area, corrosion current, equivalent weight and density of the metal involved for corrosion rate calculation in mm/year (lets say). For dissimilar metal welds, how can we measure...
30 June 2024 5,943 2 View
1- Please provide information about the chemical composition of curing agent for PDMS(Sylgard 184 Kit)? 2- what dry solid percentage is obtained for a ratio of 10 to 1(PDMS to hardener)?
30 June 2024 6,814 2 View
Respected researcher, I want to know that is there any way or one can help in generating a 2D/3D image using the energy count spectrum of from backscatter methods. How can I design such an...
29 June 2024 10,023 1 View
I do a research about Non-Verbal Communication: Gestures and Body Language in Classroom.
18 June 2024 8,737 1 View
In my XPS spectral, i detected oxygen group bonded to carbon and in my zinc spectral (note : my sample is ZnO) ...there are two valent zinc species available, (which is unsual as my previous...
17 June 2024 3,888 2 View
Action research design is Quantitative Studies or Qualitative Studies?
14 June 2024 1,684 1 View
Hi there! I was wondering how I should go about interpreting the following data. I am currently investigating the moderating role of working memory (continuous) on the relationship between...
07 June 2024 1,473 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View