Im getting a serious issue with my Plant DNA which has been degraded after several trials using 2% CTAB extraction method, the parameters I used were as followed:

Plant material: (Maple (Acer monspessulanum ) Leaf tissue conserved on siliqua gel dated at different times from most recent to 1-year storage)

CTAB buffer trials

0.2% BME - 1% PVP / 1% BME - 1% PVP / 1% BME - 5% (1H incubation at 65°C)

Chloroform IA 24:1 / Phenolo Chloroform IA 25:24:1

Precipitation on Isopropanol over night ( DNA Pellets received were white and clear not dark)

2 washings with Ethanol 70%

Centrifugation was at 13 000 rpm during the process and DNA dilution was by Ultra Pure water

(All my trials failed to produce good quality DNA and systematically prevented my PCR)