Why colour of DMF solvent changes to light brown colour on heating at above 130 C?

I was doing a blank test using DMF as solvent by heating it at 130 C. Within half an hour the colour of the solvent changes from colourless to brown colour. Is there any particular reason for...

08 September 2019 5,848 2 View

Does the integral ratio of exchangeable proptons and non-exchangeable protons matches?

While doing the NMR analyses of some polymer samples sometime I have observed that the integral ratios of some polar protons (Exchangeable protons) don't matches with the integral ratios of...

06 July 2018 1,413 7 View

How to neutralise a non polar molecule/polymer?

How a strong non polar molecule/polymer with having single or two amine/hydroxyl ions can be neutralised? Can it be done any desirable organic solvent with conc HCl? I had tried with water but...

04 May 2018 7,644 6 View

Why inhibitor are remain added in the monomer?

Most of the monomers are available with containing inhibitors! Is it just to avoid polymerization or something else?

06 July 2017 8,329 3 View

How shall I find or calculate the Gibb's free energy for a typical molecule as well as transition state from Spartan 14 wavefunction?

Hello everyone, I am using Spartan 14 wave function to calculate Gibb's free energy of a particular molecule as well as the transition state. But unfortunately, I am a bit unknown of how to use or...

04 May 2017 6,407 2 View

Polymer purification

Hi, guys! Today I am arriving with a new question. I am facing a problem while purifying a polymer. Then I doubt whether the polymer is getting purified! After synthesizing the polymer, I am...

04 May 2017 6,757 14 View

What will be the product of the 2nd reaction?

03 April 2017 8,476 8 View

Is PDMS-hydroxyl terminated soluble in DMF?

Actually, after adding PDMS (polydimethylsiloxane hydroxyl terminated Mn~600 g/mol) in DMF, it's very hard to distinguish or elucidate whether the polymer got solubilise! But I think the material...

03 April 2017 8,793 2 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

How to predict the reaction pathway for electro organic synthesis?

Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...

17 February 2021 2,383 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

How to measure acetic anhydride solution with 18M sulfuric acid?

I am doing research on the synthesis of paracetamol. I am preparing acetic anhydride solution with a few drops a sulfuric acid and wish to measure the pH of this solution. However my pH meter...

14 February 2021 8,699 3 View

Best synthesis method for large scale silver nanoparticle synthesis with high yield?.Best post processing steps after nanoparticle synthesis?

I have chemically synthesized silver nanoparticle to combat microbes on wound site. I have used many methods of synthesis, but I found minimal field of silver NP product.I am getting whitish...

11 February 2021 4,964 13 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View