In my postdoc I always used the anesthesic protocol of ketamine(80mg/kg)/xylazine (10mg/kg) injecting 0,1ml ip for balb-c mice. And it works very well.
However now using Swiss mice from another breeding this protocol is not working. We tryed a higher dose of 100mg/kg ketamine and 16mg/kg of xylazine but stil not working.
Did someone have another ketamine/xylazine protocol for swiss mice anesthesia? (I only have access of this drugs).
Thanks
Dear Dr. Bonavita, chek out for your route of administration if it does not work.
Dear Mehmet Canturk ,
thanks for the reply. as I said before, we are using the i.p. (intraperitoneal) route.
Yeserday my students tryed a bit higher dose (110mg/kg of ketamine keeping the xylazine dose) and it works well.
I'm thinking it could be the the quality of the drug I'm using.
I would argue that it may be due to the quality of your chemical. I have seen this in the past with older shelf life as well as with less quality formulations. How old is your ketamine?
Hello Renee R Donahue , I think you could be right about the quality. It is a brazilian brand but doesn't expired yet. I elevated the dose of ketamine from 100 to 130 and now is working well.
thanks fro the reply.
Glad to hear it is working for you now. Personally I love the Ketathesia brand from Henry-Schein.
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