In order to prevent premature acidification and pheophytin formation during the assay, it is advisable to add calcium carbonate to extraction solution.
hlorophylls are the green pigments in plants some of which, and perhaps all, participate in photosynthesis. An understanding of their nature, genesis, transformation, and function in plants requires that they and their near chemical relatives be recognized qualitatively and oftentimes be determined quantitatively. In addition to the use of analytical procedures for the identification and determination of the chlorophylls as participants in plant physiological processes, these methods may be used in various other fields of science and technology some of which are genetics, plant nutrition, certain aspects of pure chemistry, crop production of land and sea, medicine, pharmaceuticals, food technology, and paleontology.