Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

Explain plasmid description?

Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....

14 January 2021 6,326 2 View

How big the Balb/c mice spleen after immunization?

I am working with Balb/c mice, and in my experiment, I have to inject mice with antigens, then get their spleen and collect the splenocytes for fusion. Recently, I get into trouble with the mouse...

04 January 2021 3,591 3 View

Capture of oleophilic solid polymers present in water solution ?

I am looking for articles, coferences, publications or thesis concerning the capture of solid oleophilic polymers in solution in water. Thanks Thierry Le Blan

01 January 2021 5,798 3 View

Can I do gene expression with partial cds (not the complete one)?

Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...

27 December 2020 3,688 3 View

Hybridoma stops producing antibodies after thawing from liquid nitrogen?

Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...

01 December 2020 1,178 3 View

Suggestion for normal ovary cell lines?

Dear all, I want to choose a normal ovary cell line and check for its proliferation property when introducing carcinogenic factors. Does anyone have any suggestions for the cell line? Many thanks...

08 November 2020 9,100 3 View

What is Snapback Primer in realtime PCR?

Dear all, I find a paper using Snapback primer. I do not understand the function of snapback tail? What is snapback primer? When do we use snapback primer? and why we use that? Thank you.

01 November 2020 8,869 2 View

Rotation matrix - 3D dimension to 2D dimension?

22 October 2020 9,354 4 View

Am I introducing any potential loss of RNA integrity by combining an RNALater solution with a non-ionic detergent?

I am planning to characterise the transcriptome of pre-anthesis kiwifruit anthers. The samples are being harvested on my behalf and sent down by post, and I cannot ask the harvesters to dissect...

10 October 2020 1,830 3 View

The modern system of international relations: unipolar or multipolar world?

Do you think that there is one center of power in the modern world, or are all countries equal in the international arena?

01 January 2021 7,026 2 View

Among all the environmental pollutants globally how much % out of 100% is due to chemists?

Environmental pollutants and hazardous are major issues through which the modern world is suffering. Among all the environmental pollutants and hazardous materials how much contribution is from...

31 December 2020 6,039 3 View

What are the role of microbes for green revolution or modern advancement in science ?

We all know the role of microbes its miniature of the miracle how it can be , used in revolutionizing science in various fields.

23 December 2020 6,220 10 View

What is your opinion of "emotional intelligence" as a modern management tool?

Dear friend, what is your opinion of "emotional intelligence" as a modern management tool? Are there positive practices in your countries and in your institutions with its study and implementation?

14 December 2020 3,867 90 View

Where should I be looking for sources on the history of sharecropping in the United States?

08 November 2020 368 9 View

I need your advice about a PhD dissertation topic on Modern English Novel ?

I am studying Modern English Novel, spcifically postmodern and postcolonial novel. I am very indecisive about determining a topic. Is there anybody recommending me about a topic relating with...

05 November 2020 6,434 9 View

Does anyone have good examples of how aesthetic standards have influenced creative design in building structures?

My research is looking at the influence of creative design and technical standards on the development of architecture during a period of history. This has relevance to the modern day in terms of a...

05 November 2020 5,214 6 View

Which methods (or modern programms) are used to assess the biodiversity of highest vascular plants?

27 October 2020 240 3 View

What are Extensors of space and time in Generalized Thermodynamics?

Generalized Thermodynamics, which formulates the most universals laws of nature, assumes separation of all fields variables into conservable 'extensors' E and 'intensials' P linked by energy dU =...

20 October 2020 8,117 4 View

How the plato's curriculum theory satisfy the modern education pedagogy????

If yes or no justify

02 October 2020 3,395 3 View