I do flavonoid test for biscuit incorporated with muntingia fruit powder.
Sample extracted with metanol (1:10 ,w/v), macerated 24h, sentrifuged and filtered. Supernatan used for analysis.
I have done dpph, phenol earlier with the supernatan from the same extracts. Dpph done well. Phenol had white precipitation.
And now flavonoid have white cloudy like solution.
An aliquot supernatan, add with 0,15 ml nano2 5%, then 0,15 ml alcl3 10% and 1 ml naoh. Then read abs at 510 nm. Quercetin as standard.
But i have cloudy solution which make it difficult to read in spectro.
Do anyone could help me for these? Thanks before
https://media.neliti.com/media/publications/320063-phytochemical-and-antipyretic-potential-99216694.pdf
have you checked this?
1 mL sample + 4 mL ddH2O + 0.3 mL %5 NaNO2 then do vorteks, after 5 min add to same tube 0.6 mL %10 AlCl3 then do vorteks again, after 5 min add to same tube again 2 mL 1 mol/L NaOH + 2.1 ddH2O then do vorteks again.
I think you can get a less "cloudy" solution.
Also check the test tubes for Na2CO3 residue or other chemicals.
Thanks @HayriÜstün I forget to mention about ddH2O and vortex. Yes, I did. I add 5 ml ddH2O after sampel then vortexs for each solution added. But after NaOH I didn't add any ddH2O again as I didn't find the same prosedure about it. And before used, I usually sterilized the test tube by heating them
https://media.neliti.com/media/publications/320063-phytochemical-and-antipyretic-potential-99216694.pdf
have you checked this?
- Badges
- Science topic
- Similar topics
- Phytochemistry
- Extracts