I do flavonoid test for biscuit incorporated with muntingia fruit powder.

Sample extracted with metanol (1:10 ,w/v), macerated 24h, sentrifuged and filtered. Supernatan used for analysis.

I have done dpph, phenol earlier with the supernatan from the same extracts. Dpph done well. Phenol had white precipitation.

And now flavonoid have white cloudy like solution.

An aliquot supernatan, add with 0,15 ml nano2 5%, then 0,15 ml alcl3 10% and 1 ml naoh. Then read abs at 510 nm. Quercetin as standard.

But i have cloudy solution which make it difficult to read in spectro.

Do anyone could help me for these? Thanks before

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